egr1 egfp mice Search Results


gene exp gapdh mm99999915 g1  (Thermo Fisher)
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saline control egr1 egfp mice  (Miltenyi Biotec)
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tg(egr1-egfp)go90gsat mice  (Jackson Laboratory)
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Tg(Egr1 Egfp)Go90gsat Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ere-egfp mice  (Shanghai Model Organisms Center)
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egr-1-floxed (egr-1 f/+ ) mice  (Shanghai Model Organisms Center)
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Egr 1 Floxed (Egr 1 F/+ ) Mice, supplied by Shanghai Model Organisms Center, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd4-cre mice  (Shanghai Model Organisms Center)
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egr1-creert2 mice  (Jackson Laboratory)
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Jackson Laboratory egr1-creert2 mice
Egr1 Creert2 Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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egr1-creer t2 mice  (Jackson Laboratory)
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Jackson Laboratory egr1-creer t2 mice
Egr1 Creer T2 Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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r26rce mice (gt(rosa)26sortm1.1(cag-egfp)fsh/mmjax  (Jackson Laboratory)
90
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drd1 egfp  (Addgene inc)
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paav8-hsyn-dio-mcherry  (Addgene inc)
90
Addgene inc paav8-hsyn-dio-mcherry
Mice carrying the Egr1-CreER T2 and R26 Ai14/+ transgenes and either Drd1-EGFP or Drd2-EGFP-Rpl10a (Drd2 Trap ) transgene were microinjected in the striatum with <t>an</t> <t>AAV</t> that Cre-dependently expresses <t>mCherry.</t> Two weeks later, the mice were treated with 4-OHT (50 mg.kg −1 ) and immediately after with cocaine (20 mg.kg −1 ) or saline. A ) Confocal image in the dorsal striatum of a Drd1-EGFP mouse 1 week after a cocaine injection (20 mg.kg −1 ) showing mCherry-activity tagged cells (immunodetection, red) and EGFP fluorescence (green) corresponding to Drd1-expressing neurons. B ) Same as in A but in a Drd2 Trap mouse. A and B , scale bar: 20 µm. C ) Quantification of the number of activity-tagged neurons labeled with mCherry in mice with Drd1-EGFP or Drd2-EGFP/Rpl10a transgene, one week after saline or cocaine injection. Scatter plots with mean ± SEM. Drd1-EGFP, saline, n = 15, cocaine, n = 11, Mann-Whitney test, U = 67.5, p = 0.45; Drd2-EGFP/Rpl10a, saline, n = 8, cocaine, n = 21, Mann-Whitney test, U = 22.5, p = 0.0016 (**, see detailed statistical results in Supplementary Table 1). D ) Quantification of the percentage of EGFP-positive cells among the mCherry-tagged cells in mice with Drd1-EGFP or Drd2-EGFP-Rpl10a transgene, one week after saline or cocaine injection. Scatter plots with mean ± SEM. Drd1-EGFP, saline, n = 15, cocaine, n = 11; Student’s t test, t (24) = 5.22, p < 10 −4 , ****, Drd2-EGFP-Rpl10a, saline, n = 8, cocaine, n = 20, Student’s t test, t (26) = 0.82, p = 0.42 (see detailed statistical results in Supplementary Table 1).
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cre-inducible adeno-associated virus (aav) vectors coding for mcherry  (Addgene inc)
90
Addgene inc cre-inducible adeno-associated virus (aav) vectors coding for mcherry
Mice carrying the Egr1-CreER T2 and R26 Ai14/+ transgenes and either Drd1-EGFP or Drd2-EGFP-Rpl10a (Drd2 Trap ) transgene were microinjected in the striatum with <t>an</t> <t>AAV</t> that Cre-dependently expresses <t>mCherry.</t> Two weeks later, the mice were treated with 4-OHT (50 mg.kg −1 ) and immediately after with cocaine (20 mg.kg −1 ) or saline. A ) Confocal image in the dorsal striatum of a Drd1-EGFP mouse 1 week after a cocaine injection (20 mg.kg −1 ) showing mCherry-activity tagged cells (immunodetection, red) and EGFP fluorescence (green) corresponding to Drd1-expressing neurons. B ) Same as in A but in a Drd2 Trap mouse. A and B , scale bar: 20 µm. C ) Quantification of the number of activity-tagged neurons labeled with mCherry in mice with Drd1-EGFP or Drd2-EGFP/Rpl10a transgene, one week after saline or cocaine injection. Scatter plots with mean ± SEM. Drd1-EGFP, saline, n = 15, cocaine, n = 11, Mann-Whitney test, U = 67.5, p = 0.45; Drd2-EGFP/Rpl10a, saline, n = 8, cocaine, n = 21, Mann-Whitney test, U = 22.5, p = 0.0016 (**, see detailed statistical results in Supplementary Table 1). D ) Quantification of the percentage of EGFP-positive cells among the mCherry-tagged cells in mice with Drd1-EGFP or Drd2-EGFP-Rpl10a transgene, one week after saline or cocaine injection. Scatter plots with mean ± SEM. Drd1-EGFP, saline, n = 15, cocaine, n = 11; Student’s t test, t (24) = 5.22, p < 10 −4 , ****, Drd2-EGFP-Rpl10a, saline, n = 8, cocaine, n = 20, Student’s t test, t (26) = 0.82, p = 0.42 (see detailed statistical results in Supplementary Table 1).
Cre Inducible Adeno Associated Virus (Aav) Vectors Coding For Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Mice carrying the Egr1-CreER T2 and R26 Ai14/+ transgenes and either Drd1-EGFP or Drd2-EGFP-Rpl10a (Drd2 Trap ) transgene were microinjected in the striatum with an AAV that Cre-dependently expresses mCherry. Two weeks later, the mice were treated with 4-OHT (50 mg.kg −1 ) and immediately after with cocaine (20 mg.kg −1 ) or saline. A ) Confocal image in the dorsal striatum of a Drd1-EGFP mouse 1 week after a cocaine injection (20 mg.kg −1 ) showing mCherry-activity tagged cells (immunodetection, red) and EGFP fluorescence (green) corresponding to Drd1-expressing neurons. B ) Same as in A but in a Drd2 Trap mouse. A and B , scale bar: 20 µm. C ) Quantification of the number of activity-tagged neurons labeled with mCherry in mice with Drd1-EGFP or Drd2-EGFP/Rpl10a transgene, one week after saline or cocaine injection. Scatter plots with mean ± SEM. Drd1-EGFP, saline, n = 15, cocaine, n = 11, Mann-Whitney test, U = 67.5, p = 0.45; Drd2-EGFP/Rpl10a, saline, n = 8, cocaine, n = 21, Mann-Whitney test, U = 22.5, p = 0.0016 (**, see detailed statistical results in Supplementary Table 1). D ) Quantification of the percentage of EGFP-positive cells among the mCherry-tagged cells in mice with Drd1-EGFP or Drd2-EGFP-Rpl10a transgene, one week after saline or cocaine injection. Scatter plots with mean ± SEM. Drd1-EGFP, saline, n = 15, cocaine, n = 11; Student’s t test, t (24) = 5.22, p < 10 −4 , ****, Drd2-EGFP-Rpl10a, saline, n = 8, cocaine, n = 20, Student’s t test, t (26) = 0.82, p = 0.42 (see detailed statistical results in Supplementary Table 1).

Journal: bioRxiv

Article Title: Long-lasting tagging of neurons activated by seizures or cocaine administration in Egr1-CreER T2 transgenic mice

doi: 10.1101/2020.10.12.335745

Figure Lengend Snippet: Mice carrying the Egr1-CreER T2 and R26 Ai14/+ transgenes and either Drd1-EGFP or Drd2-EGFP-Rpl10a (Drd2 Trap ) transgene were microinjected in the striatum with an AAV that Cre-dependently expresses mCherry. Two weeks later, the mice were treated with 4-OHT (50 mg.kg −1 ) and immediately after with cocaine (20 mg.kg −1 ) or saline. A ) Confocal image in the dorsal striatum of a Drd1-EGFP mouse 1 week after a cocaine injection (20 mg.kg −1 ) showing mCherry-activity tagged cells (immunodetection, red) and EGFP fluorescence (green) corresponding to Drd1-expressing neurons. B ) Same as in A but in a Drd2 Trap mouse. A and B , scale bar: 20 µm. C ) Quantification of the number of activity-tagged neurons labeled with mCherry in mice with Drd1-EGFP or Drd2-EGFP/Rpl10a transgene, one week after saline or cocaine injection. Scatter plots with mean ± SEM. Drd1-EGFP, saline, n = 15, cocaine, n = 11, Mann-Whitney test, U = 67.5, p = 0.45; Drd2-EGFP/Rpl10a, saline, n = 8, cocaine, n = 21, Mann-Whitney test, U = 22.5, p = 0.0016 (**, see detailed statistical results in Supplementary Table 1). D ) Quantification of the percentage of EGFP-positive cells among the mCherry-tagged cells in mice with Drd1-EGFP or Drd2-EGFP-Rpl10a transgene, one week after saline or cocaine injection. Scatter plots with mean ± SEM. Drd1-EGFP, saline, n = 15, cocaine, n = 11; Student’s t test, t (24) = 5.22, p < 10 −4 , ****, Drd2-EGFP-Rpl10a, saline, n = 8, cocaine, n = 20, Student’s t test, t (26) = 0.82, p = 0.42 (see detailed statistical results in Supplementary Table 1).

Article Snippet: Cre-inducible adeno-associated virus (AAV) vectors coding for mCherry, pAAV8-hSyn-DIO-mCherry (Addgene, Cambridge, MA), were bilaterally injected into the dorsal striatum of doubly transgenic mice bearing the Egr1-CreER T2 transgene in association with Drd1-EGFP or Drd2-Rpl10aEGFP transgene.

Techniques: Injection, Activity Assay, Immunodetection, Fluorescence, Expressing, Labeling, MANN-WHITNEY